Insertion junction: LMJ.RY0402.179389_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.179389
Chromosome:chromosome 1
Location:1900170
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g010300 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGCATCCAGAGACGAGCTCTGGACGCCG
Internal bar code:TTGTCGGGCGCCACGGCACAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1070
LEAP-Seq percent confirming:99.4478
LEAP-Seq n confirming:1801
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAGCTATGCAAACCCAAT
Suggested primer 2:ACACTCATGCATTGGCACAT