| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.179395 |
| Chromosome: | chromosome 11 |
| Location: | 2832643 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477625 | CHLH2,MCH2 | Magnesium chelatase subunit H; (1 of 1) PTHR23304//PTHR23304:SF109 - SPOT2-RELATED // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAAACTCGAAGAGCATGCCGGTTGTCCC |
| Internal bar code: | CAGCGCCTTGATCCGTCAAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 402 |
| LEAP-Seq percent confirming: | 98.4117 |
| LEAP-Seq n confirming: | 3098 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTGATGGTCGATTCTCCC |
| Suggested primer 2: | CTGTACAGCGAGATGGACGA |