Insertion junction: LMJ.RY0402.179430_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.179430
Chromosome:chromosome 16
Location:602364
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g692200 MMP6 (1 of 1) IPR003609//IPR008752 - PAN/Apple domain // Peptidase M11, gametolysin; Metalloproteinase of VMP family 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGTCGACGTGGGCCCTGCGCGACACTGT
Internal bar code:GTGCCGCGCGGTGGGGAACAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:681
LEAP-Seq percent confirming:99.8016
LEAP-Seq n confirming:503
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GGACGGTCCTTGAAGAATGA
Suggested primer 2:GCTACATCCTGAACCCTGGA