| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.179460 |
| Chromosome: | chromosome_3 |
| Location: | 7876264 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g202550 | ELG11 | Exostosin-like glycosyltransferase | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGCTTTGGTACGGAACAGGACGCACCTGC |
| Internal bar code: | GCCATAGACTTGGCTTGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 227 |
| LEAP-Seq percent confirming: | 99.5586 |
| LEAP-Seq n confirming: | 5413 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTCGCTGTTGTGTTTAT |
| Suggested primer 2: | TCTATCACGAGCCGACAGTG |