| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.179480 |
| Chromosome: | chromosome 12 |
| Location: | 7145844 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559000 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAACCAAACCAACCGCGGGCAGAACTCTC |
| Internal bar code: | CCGGCTGGACAATAATTCCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 839 |
| LEAP-Seq percent confirming: | 97.6261 |
| LEAP-Seq n confirming: | 329 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGCATACCCCTTGTTCT |
| Suggested primer 2: | GAGCCGGACCATTTAACTCA |