Insertion junction: LMJ.RY0402.179495_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.179495
Chromosome:chromosome 2
Location:2853134
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g094750 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGTACGTCAGCTTTTGAGTTAGGAACTC
Internal bar code:TCGAGAGGTTGGCGCAGTCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:905
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:786
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CGTAAAGTGGCGAAGAGGAG
Suggested primer 2:CCTGTCAATGAGATGCAACG