Insertion junction: LMJ.RY0402.179516_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.179516
Chromosome:chromosome 3
Location:9009017
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g209953 (1 of 11) IPR001471//IPR016177//IPR031112 - AP2/ERF domain // DNA-binding domain // AP2-like ethylene-responsive transcription factor 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGGGCAGTATTACCGACGACCATGGTGA
Internal bar code:CGTCGCGCGGTCATGGTTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:778
LEAP-Seq percent confirming:99.4795
LEAP-Seq n confirming:1529
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTGAACGTTTCCGTTGTC
Suggested primer 2:AGGGATGGAGCAAACACAAG