Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.179520 |
Chromosome: | chromosome_10 |
Location: | 1656365 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g429750 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTTACAGTACCCGCGTGTCATGAATAGAAC |
Internal bar code: | GGATAACGCCAGGGGGAACGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 657 |
LEAP-Seq percent confirming: | 99.4604 |
LEAP-Seq n confirming: | 1106 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTACTGCCATTGTTGCTGA |
Suggested primer 2: | CATTTATGTCGTGGGCAGTG |