| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.179570 |
| Chromosome: | chromosome 14 |
| Location: | 1082092 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614850 | FAP33,ANK17 | Flagellar Associated Protein 33; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGCCGCCTGCTGCCGCCCTGCTGCCGG |
| Internal bar code: | AATAGGGTCTAAAGTCGACTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 624 |
| LEAP-Seq percent confirming: | 70.36 |
| LEAP-Seq n confirming: | 10573 |
| LEAP-Seq n nonconfirming: | 4454 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCTACTGTGTCCAGTGGT |
| Suggested primer 2: | CGTGTATGTCCCATGCGTAG |