| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.179570 |
| Chromosome: | chromosome 16 |
| Location: | 605841 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692200 | MMP6 | (1 of 1) IPR003609//IPR008752 - PAN/Apple domain // Peptidase M11, gametolysin; Metalloproteinase of VMP family | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATGGGTGTTGGTGGGGACCATGTCATTG |
| Internal bar code: | AGACAATGTCGGTGCGGAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 813 |
| LEAP-Seq percent confirming: | 99.0469 |
| LEAP-Seq n confirming: | 8314 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCCTCATAGCAGGGGTC |
| Suggested primer 2: | CCTACCTGGGTGGAGACAAA |