| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.179581 |
| Chromosome: | chromosome 1 |
| Location: | 2749588 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016300 | CAM3 | Calmodulin-like protein; (1 of 38) IPR002048//IPR011992 - EF-hand domain // EF-hand domain pair | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTCAGAAGCTTCCTCCAGCTCAGCGCAT |
| Internal bar code: | GCCAATGGAGCGTTCCAACACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 649 |
| LEAP-Seq percent confirming: | 99.4087 |
| LEAP-Seq n confirming: | 2858 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGCGTCAGCTTCTGTAAA |
| Suggested primer 2: | TTGCTGAGGCAGTACTGTGG |