Insertion junction: LMJ.RY0402.179610_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.179610
Chromosome:chromosome 9
Location:63493
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACCCGAGGTATCCCGCTGTCCCCGCCC
Internal bar code:CGGCCGCGGCTCTGGACTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:372
LEAP-Seq percent confirming:99.2137
LEAP-Seq n confirming:2902
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TACTTAGTGGGCGGGATTTG
Suggested primer 2:CCGTCGCGGTATTTTAGTGT