Insertion junction: LMJ.RY0402.179620_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.179620
Chromosome:chromosome 12
Location:8836375
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g545101 XDH1 (1 of 1) 1.17.1.4 - Xanthine dehydrogenase / Xanthine/NAD(+) oxidoreductase; Xanthine dehydrogenase/oxidase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACCAGCAGCCCTTCACCTTTTCACATTC
Internal bar code:AGACAACAGCAATGAAAGAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:788
LEAP-Seq percent confirming:99.898
LEAP-Seq n confirming:979
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CGTAATACGCAAGCGACTCA
Suggested primer 2:GCGACCTTCTGTGTGCTGTA