| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.179638 |
| Chromosome: | chromosome 12 |
| Location: | 7732343 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553900 | CNG2 | (1 of 1) K04905 - potassium voltage-gated channel Eag-related subfamily H member 2 (KCNH2); Cyclic-nucleotide gated potassium channel | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGAACCACATTTTCTGCGGCGCGCAACAG |
| Internal bar code: | GATTTGTGTGCGCCCTATCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1126 |
| LEAP-Seq percent confirming: | 99.6842 |
| LEAP-Seq n confirming: | 4103 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTGTAAGTACCAGCCCA |
| Suggested primer 2: | GAGAGAGAGACCGTTGGTGC |