| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.179639 |
| Chromosome: | chromosome 4 |
| Location: | 3417070 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g227600 | (1 of 1) PTHR27000:SF77 - LEUCINE-RICH REPEAT PROTEIN KINASE-LIKE PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTGCCGCCTGGCAGCAATGTGTTCGTG |
| Internal bar code: | AAAGAGTACAGATTTTTATGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 48 |
| LEAP-Seq percent confirming: | 90.3226 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCGTCCTGGAGTCAAAAT |
| Suggested primer 2: | AGCCGTACAACCGTACAACC |