| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.179683 |
| Chromosome: | chromosome 6 |
| Location: | 2396646 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268050 | HTA10 | Histone H2A; (1 of 30) K11251 - histone H2A (H2A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGTGCAGCAGGGTATCAGGTATGCCCA |
| Internal bar code: | ACAGACGAACCAGCTACTGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1049 |
| LEAP-Seq percent confirming: | 99.5861 |
| LEAP-Seq n confirming: | 1203 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAAGAAGGGCAAGTACGC |
| Suggested primer 2: | CGGGGGTCCCTAAGAGTAAG |