Insertion junction: LMJ.RY0402.179706_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.179706
Chromosome:chromosome 16
Location:563400
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g692450 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACGCCACCACGGACGCCGCCAGCAGCAG
Internal bar code:GTACAGGTCCGCGACCTTATTC

Confirmation - LEAP-Seq

LEAP-Seq distance:568
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGTAGGTGGACTGGAGGAGG
Suggested primer 2:CTTATCCCCCAAAGGACCAT