| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.179731 |
| Chromosome: | chromosome 6 |
| Location: | 3049298 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274400 | SMM19 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) 2.1.1.244 - Protein N-terminal methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTAGGAGGGAGGGCGGCGGGCGCGGGC |
| Internal bar code: | TCCTCTGCGCCCAAACCAAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 402 |
| LEAP-Seq percent confirming: | 97.2703 |
| LEAP-Seq n confirming: | 1853 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGGGTCGTGTGACTGAG |
| Suggested primer 2: | CCTTCTCGAACAAGTCCAGC |