| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.179769 |
| Chromosome: | chromosome_5 |
| Location: | 1320950 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g244800 | NUOAF3 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGTCCTGCATCCCGTGCCTCGTCCTGCCT |
| Internal bar code: | CCTCGGATCAACCAGACTTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 200 |
| LEAP-Seq percent confirming: | 79.8165 |
| LEAP-Seq n confirming: | 435 |
| LEAP-Seq n nonconfirming: | 110 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTAGCCGTTCATGTAAA |
| Suggested primer 2: | GAGGGGGAGTGTATTGGGAT |