| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.179793 |
| Chromosome: | chromosome 16 |
| Location: | 2935069 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g664050 | (1 of 1) PF02893//PF16016 - GRAM domain (GRAM) // Domain of unknown function (DUF4782) (DUF4782) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGTAAACAGTACTGCAGACTATAGACAG |
| Internal bar code: | CCCCTCGTGGAGTTCATCTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 48 |
| LEAP-Seq percent confirming: | 99.5392 |
| LEAP-Seq n confirming: | 216 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCTCATGTGAGAGCAGGG |
| Suggested primer 2: | TTTTACAGGTGTGCAGCGTC |