Insertion junction: LMJ.RY0402.179795_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.179795
Chromosome:chromosome 7
Location:1719044
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g325711 (1 of 2) K19363 - lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACGCGTCCGTGCCACCTACTGTTGCAAT
Internal bar code:GGGAACGATCAGGCCCGCAAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:640
LEAP-Seq percent confirming:99.6301
LEAP-Seq n confirming:808
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCTAAATGCTGCTCCTCACC
Suggested primer 2:AACCTTTGGAATGAAACCCC