| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.179846 |
| Chromosome: | chromosome 10 |
| Location: | 4945539 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455050 | (1 of 1) IPR000104//IPR003347//IPR006917 - Antifreeze protein, type I // JmjC domain // SOUL haem-binding protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTCTCGAGGTCATGAACGGGCGGCCCG |
| Internal bar code: | ACAACCACTTGAGTGGCAATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 435 |
| LEAP-Seq percent confirming: | 99.0191 |
| LEAP-Seq n confirming: | 4139 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTTTGACCCTTGTAGGC |
| Suggested primer 2: | CAGCTTGATGCAGGTCTTGA |