| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.179857 |
| Chromosome: | chromosome 10 |
| Location: | 3714389 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446275 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGTTGCCACCAAGCACCGCGGCGATCAC |
| Internal bar code: | TCAGGCACGCGGTAGGTATTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 737 |
| LEAP-Seq percent confirming: | 99.5958 |
| LEAP-Seq n confirming: | 1725 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCGTCTTCAATCCCGATG |
| Suggested primer 2: | CATACAACCGCTTCACCTCC |