| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.179895 |
| Chromosome: | chromosome 12 |
| Location: | 1238203 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489450 | (1 of 2) IPR017993 - Atrophin-1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGGCGTTGTACCAGCCGCCTCATGCCTT |
| Internal bar code: | GCTCTCCTGAACTCTCGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 985 |
| LEAP-Seq percent confirming: | 99.507 |
| LEAP-Seq n confirming: | 3431 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTAGAGGGTGGTAGTGCC |
| Suggested primer 2: | ATATGCGGCAACCGAACTAC |