Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.179904 |
Chromosome: | chromosome 2 |
Location: | 7558616 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g143850 | (1 of 8) PF00514 - Armadillo/beta-catenin-like repeat (Arm) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACTGGTCTGCTGGTACGGCGACACCGCT |
Internal bar code: | ACTACCTTCCTAAGATTCGGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 185 |
LEAP-Seq percent confirming: | 99.7907 |
LEAP-Seq n confirming: | 3337 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCCCACACTCCTATTCATT |
Suggested primer 2: | CTCTTACAGGAAGTTGCGGC |