| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.179908 |
| Chromosome: | chromosome 17 |
| Location: | 2425619 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g715600 | (1 of 1) PF11822 - Domain of unknown function (DUF3342) (DUF3342) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCAGTAAGGCTGAGGCCGTTTCCCGC |
| Internal bar code: | GATCGCTCTGTCCCGCCAGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 97 |
| LEAP-Seq percent confirming: | 99.845 |
| LEAP-Seq n confirming: | 644 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACGTGCCAGTAGATGTCG |
| Suggested primer 2: | CACCTCCGCATAAACCAACT |