Insertion junction: LMJ.RY0402.179928_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.179928
Chromosome:chromosome_8
Location:4189779
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre08.g380151 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):GGTTGGGCGGGGTCGGGCGGCACGGTCACG
Internal bar code:CGAAACGCGTGGCCTGTTGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:45
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:394
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTATCTACAACGCACCCA
Suggested primer 2:AAGGAGAAGGACACGCAGAA