| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.179946 |
| Chromosome: | chromosome 13 |
| Location: | 4180345 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g592200 | GLT1,GSN1 | Glutamate synthase, NADH-dependent; (1 of 1) 1.4.1.14 - Glutamate synthase (NADH) / NADH-glutamate synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTAGATTACGGTTTCGCAGCCTAGGTA |
| Internal bar code: | TCCGGCGTTAAGGGCAATGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1035 |
| LEAP-Seq percent confirming: | 99.1848 |
| LEAP-Seq n confirming: | 1095 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTGATGGAGGCAGGCTAC |
| Suggested primer 2: | CTTGCCCCTCGCTACTACTG |