Insertion junction: LMJ.RY0402.179949_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.179949
Chromosome:chromosome 1
Location:4583806
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g031350 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGACGCGGCGCTCTGAGCGCGTGGGGGGA
Internal bar code:TTATACGGTGCGCCTCCGTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:637
LEAP-Seq percent confirming:99.8653
LEAP-Seq n confirming:5191
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTGTCATCTCCGCTAATG
Suggested primer 2:AAGGTCGGTTGTGGACTCAC