| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.179954 |
| Chromosome: | chromosome 4 |
| Location: | 3866737 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g230340 | FAP206 | (1 of 1) PTHR21442:SF0 - UPF0704 PROTEIN C6ORF165; Flagellar Associated Protein 206 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCAGAGGCTGCTGAGGGACATCAGCAGG |
| Internal bar code: | ACCGTCGGGGATCGGCATGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 12.0879 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 560 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCTTTGCGCAATGTTTTC |
| Suggested primer 2: | CGGTGTGTGTGTGTGTGTGT |