| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.179960 |
| Chromosome: | chromosome 6 |
| Location: | 3055537 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274450 | SMM20 | (1 of 5) PTHR13271//PTHR13271:SF28 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // SUBFAMILY NOT NAMED; S-adenosyl-L-methionine-dependent methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCACTTCCCATGGTTAAGGCAGCAGGAG |
| Internal bar code: | AAAGAAGTGTTTTGTTATCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 814 |
| LEAP-Seq percent confirming: | 99.5284 |
| LEAP-Seq n confirming: | 6754 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAATGTTTCTCGTTGAGCG |
| Suggested primer 2: | GACTTCACGAACCTGGTGGT |