| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.179964 |
| Chromosome: | chromosome 3 |
| Location: | 2315719 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g158300 | PTK7 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK; Putative tyrosine kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTTGCCCCCTCCTCCCCTGCCTGACCT |
| Internal bar code: | TATCTTGGGCTCGGCTATAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1008 |
| LEAP-Seq percent confirming: | 99.5406 |
| LEAP-Seq n confirming: | 1300 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGCACAGGGAATGAAGC |
| Suggested primer 2: | GGTGGGAGCTGAATGTTTGT |