Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.179997 |
Chromosome: | chromosome_8 |
Location: | 3500071 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g375050 | FAP86 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GAAACAGCACTCTTACGCTGTATTACTCTC |
Internal bar code: | TGTTGGTGTCTTGCTGATACG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 646 |
LEAP-Seq percent confirming: | 99.7779 |
LEAP-Seq n confirming: | 1797 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACTTGGAGCCGATGAGCTT |
Suggested primer 2: | ACTTAGTCGCGTCGTCGTTT |