| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.180002 |
| Chromosome: | chromosome 16 |
| Location: | 2980771 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g664350 | CLD1 | (1 of 1) PTHR10992//PTHR10992:SF752 - ALPHA/BETA HYDROLASE FOLD-CONTAINING PROTEIN // ALPHA/BETA-HYDROLASE-LIKE PROTEIN; Putative Chlorophyll dephytylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGTGACTGGCGGACCATGAGCATATTCA |
| Internal bar code: | GTGCTCGGCACGGGGTGGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 967 |
| LEAP-Seq percent confirming: | 98.5835 |
| LEAP-Seq n confirming: | 3271 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATGTCGCGTTACTTCTGC |
| Suggested primer 2: | TGGCTGGGGTTTATTTCAAG |