| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.180028 |
| Chromosome: | chromosome 7 |
| Location: | 4906311 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346317 | GT90F39,GT90-39 | GT90 family protein 39; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGGCAGCTAGGGGGGAGGGTGCGGGTAC |
| Internal bar code: | AAGCGGCTGGTCTTCTCCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 779 |
| LEAP-Seq percent confirming: | 93.4555 |
| LEAP-Seq n confirming: | 20449 |
| LEAP-Seq n nonconfirming: | 1432 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCGCAAACGCAGAATTAG |
| Suggested primer 2: | GCGTGTGTGTGTGTGTGTGT |