| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.180051 |
| Chromosome: | chromosome 1 |
| Location: | 5132970 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035700 | (1 of 781) IPR000104 - Antifreeze protein, type I | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCAAATCGAAAACCCATACGACACGTGA |
| Internal bar code: | GACCGCGTCGGAGTTGTCCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 747 |
| LEAP-Seq percent confirming: | 99.1304 |
| LEAP-Seq n confirming: | 798 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTAAAATGGTGGGTATCG |
| Suggested primer 2: | CAGTACCACCTGCAGCAAAA |