| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.180058 |
| Chromosome: | chromosome 1 |
| Location: | 4553759 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g031050 | (1 of 1) K15172 - transcription elongation factor SPT5 (SUPT5H, SPT5) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGGCAGCTGGGCGCGCTACACGCCCGC |
| Internal bar code: | ATGATGACGCCATCGCGGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 38 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAATGAGTAGTGGCGGTGT |
| Suggested primer 2: | TACGACAGGGCAAGGGTTAC |