| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.180063 |
| Chromosome: | chromosome 6 |
| Location: | 5543496 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g285150 | APx-R,APX2 | Ascorbate peroxidase-related; (1 of 3) 1.11.1.11 - L-ascorbate peroxidase / Ascorbic acid peroxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAAGGTGCGTGAGAGGGTGCGAGTTACG |
| Internal bar code: | CCTGACCGGACGCGTGCTGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 317 |
| LEAP-Seq percent confirming: | 95.2341 |
| LEAP-Seq n confirming: | 11330 |
| LEAP-Seq n nonconfirming: | 567 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGTGCATGTGTGTGTGT |
| Suggested primer 2: | GAGTACAAGCGTCCTTTCGC |