| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.180064 |
| Chromosome: | chromosome 16 |
| Location: | 5992750 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676600 | (1 of 4) IPR029041 - FAD-linked oxidoreductase-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGTGCGTGTCAGGCTCACACCCGGTCC |
| Internal bar code: | TTATCTCGAGTATGACTAAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 894 |
| LEAP-Seq percent confirming: | 98.2573 |
| LEAP-Seq n confirming: | 5920 |
| LEAP-Seq n nonconfirming: | 105 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGGACACTAACCCTGGA |
| Suggested primer 2: | CAGACTGACCCAGAACGACA |