| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.180070 |
| Chromosome: | chromosome 12 |
| Location: | 2144744 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508853 | BPL2 | Biotin-protein ligase; (1 of 41) PF13920 - Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGTTTTACATATGCGGTCCACAGGCTA |
| Internal bar code: | AGGTGCACGGTGGTGCGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 734 |
| LEAP-Seq percent confirming: | 98.4804 |
| LEAP-Seq n confirming: | 2009 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCACCCTCTCTGAGCAAC |
| Suggested primer 2: | ACAGCAATCTCGCAGGACTT |