Insertion junction: LMJ.RY0402.180095_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.180095
Chromosome:chromosome 3
Location:3197407
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g165450 (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCGTCGTCTGTCACGATGACAAGGAAAC
Internal bar code:ACGCCGACTCTAACGCGGCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1227
LEAP-Seq percent confirming:99.5098
LEAP-Seq n confirming:203
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GAGAGAGGAACGATGCTTGG
Suggested primer 2:GTGGCCCTTTCTAGGGTTTC