Insertion junction: LMJ.RY0402.180104_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.180104
Chromosome:chromosome_13
Location:3119245
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g584901 sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TTGAGTACAAGATCAAGCACGTAGAGGAGG
Internal bar code:CGGCGCACCAGGTCCGTCCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:457
LEAP-Seq percent confirming:99.7099
LEAP-Seq n confirming:1031
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTACTGCCACTGCTACTG
Suggested primer 2:TGCACCTACCTACCCACACA