| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.180117 |
| Chromosome: | chromosome_13 |
| Location: | 2514566 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| None |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TACGTAGGCGGTTGGCACGTGTCACCCCCT |
| Internal bar code: | AAGAAGAGGGTTGTCGCAATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 218 |
| LEAP-Seq percent confirming: | 99.3039 |
| LEAP-Seq n confirming: | 856 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGTCCTCATCTCCATGT |
| Suggested primer 2: | GGTGAGCTTTGGCTCAGTTC |