| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.180165 |
| Chromosome: | chromosome 17 |
| Location: | 3692852 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726950 | (1 of 1) PTHR11042//PTHR11042:SF102 - EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED // INTERFERON-INDUCED, DOUBLE-STRANDED RNA-ACTIVATED PROTEIN KINASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGGGAGCTTGGCAGCGACTCGGACGGCG |
| Internal bar code: | AGGATCTCTCTGCCTGGGCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 148 |
| LEAP-Seq percent confirming: | 99.1534 |
| LEAP-Seq n confirming: | 1874 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTACTGGTGCTGGTGGTA |
| Suggested primer 2: | CACATCCTTTCGTGCTTTCA |