| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.180166 |
| Chromosome: | chromosome 2 |
| Location: | 5141129 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGATTTCAACATGCGTCGTCGATGCCGCC |
| Internal bar code: | AGATCGTCGACTGCCTTGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 527 |
| LEAP-Seq percent confirming: | 99.5856 |
| LEAP-Seq n confirming: | 3364 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACACAGCTACACCGCAAGG |
| Suggested primer 2: | TACATGCGGAGCTCAAACAC |