| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.180175 |
| Chromosome: | chromosome 12 |
| Location: | 3984135 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516700 | 5'UTR_intron | ||
| Cre12.g516717 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTTAGCGAATGATGAATGTCCGTTGTTG |
| Internal bar code: | ACCGCGAGTTAAGGCAATAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 726 |
| LEAP-Seq percent confirming: | 94.4131 |
| LEAP-Seq n confirming: | 1504 |
| LEAP-Seq n nonconfirming: | 89 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTAGGCACTTTCCCTTCCC |
| Suggested primer 2: | GCACGCCTAGTCAAGAGGAC |