| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.180186 |
| Chromosome: | chromosome 9 |
| Location: | 1965772 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394850 | TEF24 | LrgB-like protein; (1 of 1) PTHR30249 - PUTATIVE SEROTONIN TRANSPORTER | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCGCGCGGCAAACCAACCGCCTGGCCTG |
| Internal bar code: | GGTGGGGCATTGAAACCATTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 866 |
| LEAP-Seq percent confirming: | 84.1351 |
| LEAP-Seq n confirming: | 5457 |
| LEAP-Seq n nonconfirming: | 1029 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGTCGGTGACAGGATGT |
| Suggested primer 2: | AAAGGGGTTGCCAATTTACC |