| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.180189 |
| Chromosome: | chromosome 10 |
| Location: | 3952134 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448500 | (1 of 3) 3.5.1.110 - Peroxyureidoacrylate/ureidoacrylate amidohydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTATCATGGCCTCTGACATAGCATTGCA |
| Internal bar code: | TATTTTCACGAACTACGTATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1145 |
| LEAP-Seq percent confirming: | 95.4545 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAATACCATACGCATCAC |
| Suggested primer 2: | CACATTACGCTCACACCACC |