| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.180201 |
| Chromosome: | chromosome 2 |
| Location: | 5701926 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110050 | (1 of 1) PTHR22844:SF160 - WD40 REPEAT-CONTAINING PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTATATACTTCTGCGTGTTTCCTTGCTA |
| Internal bar code: | CAGGAAACGAGGGGTAACGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 529 |
| LEAP-Seq percent confirming: | 99.9028 |
| LEAP-Seq n confirming: | 2055 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGTCTCCAAGCGTAAAG |
| Suggested primer 2: | ATGACAGCTTGATTGGGACC |