| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.180215 |
| Chromosome: | chromosome 2 |
| Location: | 7436126 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g144500 | (1 of 5) PTHR10288 - KH DOMAIN CONTAINING RNA BINDING PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAAACGGGCCGCGGCCCTTTCCTCCGTT |
| Internal bar code: | CTCGGAGCTGTGTACTTTGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 590 |
| LEAP-Seq percent confirming: | 97.5011 |
| LEAP-Seq n confirming: | 9091 |
| LEAP-Seq n nonconfirming: | 233 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCACGACTGCTTACGGTAT |
| Suggested primer 2: | GCTGGCTACTAGATCGTCCG |